Amphibian decline has become more pervasive over the last 20 years. This decline has been sparked by a number of extrinsic factors ranging from habit destruction to emerging pathogens. Ranaviruses are a group of these pathogens responsible for triggering a number of die-offs amongst native and aquacultured fish and amphibian populations. Our primary focus is the elucidation of virus gene function facilitated by antisense technologies in an attempt to further understand the molecular mechanisms involved in replication, virulence, and pathogenesis. By understanding these events we hope to provide the foundations needed to slow and possibly abrogate future ranavirus associated mass mortalities among already threatened amphibian populations.